problem in biom convert from text file to biom file

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Ping

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Jun 26, 2017, 4:07:08 PM6/26/17
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Hello,


I would like to convert an OTU table to BIOM format. I run the following command. There was no error, but the command did not run and did not generate a biom file.

The content of the text file is as follows:
# Constructed from biom file
#OTU IDBS_0_1_1BS_0_1_2BS_0_1_3BS_0_2_1
OTU00010208266248
OTU00023343577525654065
OTU0003269751176148259934
OTU0004132715389062
OTU0005154802710
OTU0006001560
OTU0007140371884325166
OTU000814333415095
OTU00096488312422212348
OTU0010482692947688
.......................................................


BS_X_X_X is sample id. There is only count information for each OTU and no taxonomy column.

Does anybody know how to convert the text file to a biom file?

Thank you.

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Se Jin Song

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Jun 26, 2017, 7:10:20 PM6/26/17
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Hi Ping,

You're just missing one dash.

Pass
--to-hdf5 
instead of 
-to-hdf5

Best,
Se Jin

Ping

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Jun 26, 2017, 7:16:28 PM6/26/17
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Hi Se Lin,

Thanks for your reply. I changed "-" to "--", but it still did not run. BTW, I am using Qiime 1.9.1.

Ping

Se Jin Song

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Jun 26, 2017, 7:28:54 PM6/26/17
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Can you try 
--to-json
instead?

Thanks,
Se Jin

Ping

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Jun 26, 2017, 7:39:22 PM6/26/17
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It did not work either. Not sure why it did not run.

Ping

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Jun 27, 2017, 12:03:40 PM6/27/17
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The problem was the quotation mark. It works after I revised it.

Cheers,
Ping


Se Jin Song

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Jun 27, 2017, 1:00:31 PM6/27/17
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Hi Ping, 
Glad you were able to figure it out!  Thanks for posting it.

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