In "split_libraries_fastq.py", what is "barcode_type"?

[Yiming Meng]

Hi everyone,

I'm doing some paired end sequencing and I have a silly question. 

What is "barcode_type" in "split_libraries_fastq.py"? Is that the length of the barcodes of my samples?

Thanks a lot!

Yiming

[Red]

Hi Yiming,

With the barcode type option you can specify what type of barcodes you have used in your sequencing.
Yes, this can also be the length of the barcodes (for example if all your barcodes consists of 6 nucleoides, you want to pass 6 for this option.
If you did not used any barcodes at all (for example, the sequencing centre already demultiplexed the sequences for you and took out the barcodes off your sequences), then pass “not-barcoded” for this option

Red