How big is a fish?
Usually, interaction with the persistent datastore is the main factor in web app performance, unless your code does some really terrible things.
Getting a connection takes the longest, so you want connection pooling.
After that your indexing scheme has to be right. Too many indexes and your inserts and updates become slow. Not enough indexes and your selects become slow.
Cached data and/or cached pages are the fastest.
If I understand the Fine Pyramid Manual, traversal means using Zope while url dispatch means using a relational db. A little research might give you some insight into the relative performance of the two.
Get the code working first, then worry about performance.