Do you have more than one app? that might be a bit tricky -- or might not -- I think a Django app is essentially a Python package -- so you should be able to bundle it up with PyInstaller just like any other app.
But Django needs a web server to run behind:
I suggest that you use one of the Python servers, such as gunicorn:
The you'll need a python start up script that fires up gnuicorn. That start up script will be the "Application" as far as PyInstaller is concerned.
Then you'll "just" need to make sure PYInstaller bundles up all the code and data you need to run your app.
-CHB