Addition of Cellular prevalance in single sample not add up to 1?

[Nilesh Gardi]

Hello,

    We have longitudinal samples from the same patient. Samples were collected over time under the pressure of chemotherapies. We infer the clonality using the PyClone tool and are satisfied with the results. However, we had a question why the addition of Cellular prevalence values in a single sample does not add up to 1? Please see the below table for reference.

Columns depict samples (collected from the same patient over time) and rows represent clones identified from the PyClone tool. Values in each cell represent cellular prevalence (CP) values. My question is why do the addition of CP in Biopsy (or in Surgery) samples not add up to 1? Though, my Biopsy and Surgery samples were FFPE in nature, while Rec_1, Rec_2, and Rec_3 were fresh-frozen.

Let me know the 

Please guide.