Jef, PTGui is not able to level the panorama automatically without vertical and/or horizontal control points or orientation data from the camera. The best it can do is straighten it by attempting to equalise the pitch and roll values. If the axis of rotation of the panorama head was was precisely vertical, this process should then also level the panorama. Or, If you were to precisely level the camera itself for the first shot, then you could use a template which set pitch and roll of the first image to 0 and thereby produce a levelled panorama automatically. Note that the edit menu of the Panorama Editor contains both options: Level (using vertical and horizontal line points) and Straighten.
John