Hi Martin,
Are you using pyo for sound? (see preferences > general > audioLib.) I
mocked up an example, and it sounds pretty reasonable to me using
1.76, on mac 10.8, using pyo. Caveat: I have absolutely zero musical
training.
I made a routine with a default sound (onset 0.0, duration 0.1s, and a
text stim which displays a space (i.e., nothing) for 0.4s. I enclosed
this routine in a loop, for 20 iterations. The short tone is generated
by psychopy, not read in from a file, but I think that should not
matter much). 0.4s is the ITI, and using a variable should work there
just fine.
This is pretty quick and dirty, and timing might be off by 16.67ms.
Using code to interface with pyo directly you should be able to get
very precise control over the sound, in ways that are not possible
(yet?) through the Builder interface.
--Jeremy
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