New ProteomeXchange dataset PXD068411

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Dear ProteomeXchange subscriber, a new ProteomeXchange dataset is being announced. To see more information, click here:

https://proteomecentral.proteomexchange.org/dataset/PXD068411

Summary of dataset

Status: new
Identifier: PXD068411
HostingRepository: PRIDE
Species: Mus musculus
Title: Reduction of charged glycoconjugates in TANGO2 KO mouse brain
Submitter: Timothy McCubbin
LabHead: Nicolle H. Packer
Description: The transport and Golgi organization 2 (TANGO2) gene has been identified to cause a rare recessive genetic disorder known as TANGO2-deficiency disorder (TDD). TDD is a multisystemic disorder that can affect different organs including the brain, heart, and muscles, presenting as recurrent metabolic crises, muscle weakness, and neurological symptoms including developmental delay and seizures. In a TANGO2-KO Cal51 cell line model, N-glycomics of the TANGO2-KO cell line showed a significant change in glycomics profile, that was also corroborated with RNAseq transcriptomics showing increased mRNA levels of the respective glycosyltransferases. To investigate the effects of TANGO2-KO on the glycans in the brain of a mouse model, we profiled the polysialic acid (polySia), glycosaminoglycans (GAG), glycolipid glycans (GSL), and protein N- and O-glycans (NG, OG) of the cortex of the TANGO2-KO mice using the Same Sample Sequential Multi-Glycomics workflow. Overall, we observed a se
vere decrease in chondroitin sulfate GAG disaccharides, and a mild decrease in sialylation across GSL, NG and OG, resulting in a broad decrease in negatively charged glycoconjugates. When comparing against an independent DDA LFQ proteomics acquisition of the same mice model, amongst the glyco-enzymes, only sialic acid synthase (NANS) and chondroitin polymerising factor (CHPF) was also found to be significantly down regulated in the brain, correlating with the changes observed by the multi-glycomics analysis. This overall decrease in negatively charged glycoconjugates due to the loss of TANGO2 function might thus relate to the neurological impairments of TDD.

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