New ProteomeXchange dataset PXD069335

[ProteomeCentral Agent]

Dear ProteomeXchange subscriber, a new ProteomeXchange dataset is being announced. To see more information, click here:

https://proteomecentral.proteomexchange.org/dataset/PXD069335

Summary of dataset

Status: new
Identifier: PXD069335
HostingRepository: PRIDE
Species: Homo sapiens
Title: Low-resolution FAIMS for increased peptide coverage in low-load and single-cell proteomics
Submitter: Manuel Matzinger
LabHead: Karl Mechtler
Description: Field Asymmetric Ion Mobility Spectrometry (FAIMS) is an indispensable tool in single-cell and low-input proteomics. Here, we show that tuning FAIMS resolution via electrode temperature modulation improves sensitivity. We demonstrate that lowering FAIMS resolution broadens the compensation voltage window, thereby increasing ion transmission. This significantly improves ion counts, quantitative precision and peptide identifications by 25 - 34%. These results were mirrored when analyzing single cell samples.

HTML_URL: https://proteomecentral.proteomexchange.org/dataset/PXD069335
XML_URL: https://proteomecentral.proteomexchange.org/dataset/PXD069335&outputMode=XML