New ProteomeXchange dataset PXD059029

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https://proteomecentral.proteomexchange.org/dataset/PXD059029

Summary of dataset

Status: new
Identifier: PXD059029
HostingRepository: jPOST
Species: Homo sapiens
Title: Protein expression in warmed human lens epithelium cells using shotgun proteomics
Submitter: Hiroko Otake
LabHead: Noriaki Nagai
Description: In previous studies, we reported that cumulative thermal dose estimation in crystalline lens, as determined through computer simulation analysis using a supercomputer and a biothermal transport equation, showed a strong correlation with the prevalence of nuclear cataracts. In this study, we have investigated what kind of protein expressed underlying normal (35.0 °C) and warming conditions (37.5 °C) by using the shotgun Liquid chromatography with tandem mass spectrometry (LC-MS/MS)-based global proteomic approach. A total of 615 proteins were identified in the iHLEC-NY2, including 307 (49.9%) present in both lens cultured at 35.0 °C and 37.5 °C, 130 (21.1%) unique to lens cultured at 35.0 °C, and 178 (29.0%) unique to lens cultured at 37.5 °C. Furthermore, we have discussed the changes in protein expression in warmed iHLEC-NY2 cells using Gene Ontology analysis and a label-free semiquantitative method based on spectral counting. Shotgun proteomic analysis revealed that wa
rming decreased the expression of actin, alpha cardiac muscle 1, actin-related protein 2, putative tubulin-like protein alpha-4B, ubiquitin carboxyl-terminal hydrolase 17-like protein 1, ubiquitin-ribosomal protein eL40 fusion protein, ribosome biogenesis protein BMS1 homolog, histone H2B type 1-M, and histone H2A.J. in iHLEC-NY2. Thus, the decrease in specific protein levels of actin, tubulin, ubiquitin, ribosomes, and histones may be related to cataract development under warming conditions. This study may serve as a valuable indicator for elucidating the relationship between temperature and onset of nuclear cataracts.

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