New ProteomeXchange dataset PXD068076

[ProteomeCentral Agent]

Dear ProteomeXchange subscriber, a new ProteomeXchange dataset is being announced. To see more information, click here:

https://proteomecentral.proteomexchange.org/dataset/PXD068076

Summary of dataset

Status: new
Identifier: PXD068076
HostingRepository: PRIDE
Species: Homo sapiens
Title: Induced pluripotent stem cell-derived macrophages as a model for human inflammasome signalling
Submitter: Mohammad Arefian
LabHead: Ben C. Collins
Description: Macrophage models are a mainstay of inflammasome research, however current human in vitro macrophage models have significant limitations. Here we generate induced pluripotent stem cell (iPSC)-derived macrophages (iMacs) to study inflammasome signalling and benchmark them with human monocyte-derived macrophages (HMDMs). We confirm that iMacs express high levels of macrophage markers and are highly phagocytic. Whole cell proteomics analysis shows that iMacs express many inflammasome sensors and related proteins, and in functional assays iMacs respond to multiple inflammasome stimuli. The NLRP3 inflammasome is strongly activated in iMacs and we find that nigericin alone activates NLRP3. The non-canonical inflammasome does not require a priming step in iMacs as caspase-4 is constitutively expressed. High levels of NAIP/NLRC4 inflammasome activation are also observed in response to needle toxin. Finally, unlike HMDM, iMacs activate NLRP1. Therefore, we demonstrate that iMacs
are a physiologically relevant and highly tractable model to study human inflammasome signalling and regulation.

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