New ProteomeXchange dataset PXD073144
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5:55 AM (8 hours ago) 5:55 AM
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Dear ProteomeXchange subscriber, a new ProteomeXchange dataset is being announced. To see more information, click here:
https://proteomecentral.proteomexchange.org/dataset/PXD073144
Summary of dataset
Status: new
Identifier: PXD073144
HostingRepository: PRIDE
Species: Trichoderma atroviride, Cadophora luteo-olivacea
Title: Unveiling Oak Endophyte Dynamics: Cadophora luteo-olivacea and Trichoderma atroviride from Pathogenicity Tests to Omics Insights
Submitter: Martin Cerny
LabHead: Martin Cerny
Description: Pedunculate oak (Quercus robur) is a foundation tree species in European forests and reforestation programs, and forest nurseries represent key phytosanitary bottlenecks where asymptomatic planting stock can disseminate cryptic pathogens. Cadophora luteo-olivacea is frequently associated with grapevine trunk diseases and has been recovered from diverse woody hosts, yet its pathogenic potential on oak and its interactions with antagonistic fungi remain unresolved. Here, we combine pathogenicity testing, dual-culture confrontation assays and time-resolved, spatially resolved contact-zone proteomics and metabolomics to connect detection to causality and mechanism in an oak associated Cadophora–Trichoderma system. We fulfill Koch’s postulates for C. luteo-olivacea on Q. robur seedlings and quantify inhibition of Cadophora by a natural isolate of Trichoderma atroviride. Contact-zone proteomics at 4 and 8 days post-contact reveals a staged, interface-localized antagonist
ic program dominated by fungal cell-wall targeting hydrolases (including chitinases and β-1,3-glucanases), secreted proteases, oxidoreductases, transporters and small secreted proteins, alongside factors consistent with adhesion and self-protection. Together, these complementary assays provide the first experimental evidence that C. luteo-olivacea is pathogenic on Q. robur and delineate mechanistic signatures of T. atroviride mycoparasitism at the interaction front. This framework links nursery-relevant pathogen screening to mechanistic readouts and informs evaluation of biocontrol potential in a foundation forest species.
HTML_URL: https://proteomecentral.proteomexchange.org/dataset/PXD073144
XML_URL: https://proteomecentral.proteomexchange.org/dataset/PXD073144&outputMode=XML
https://proteomecentral.proteomexchange.org/dataset/PXD073144
Summary of dataset
Status: new
Identifier: PXD073144
HostingRepository: PRIDE
Species: Trichoderma atroviride, Cadophora luteo-olivacea
Title: Unveiling Oak Endophyte Dynamics: Cadophora luteo-olivacea and Trichoderma atroviride from Pathogenicity Tests to Omics Insights
Submitter: Martin Cerny
LabHead: Martin Cerny
Description: Pedunculate oak (Quercus robur) is a foundation tree species in European forests and reforestation programs, and forest nurseries represent key phytosanitary bottlenecks where asymptomatic planting stock can disseminate cryptic pathogens. Cadophora luteo-olivacea is frequently associated with grapevine trunk diseases and has been recovered from diverse woody hosts, yet its pathogenic potential on oak and its interactions with antagonistic fungi remain unresolved. Here, we combine pathogenicity testing, dual-culture confrontation assays and time-resolved, spatially resolved contact-zone proteomics and metabolomics to connect detection to causality and mechanism in an oak associated Cadophora–Trichoderma system. We fulfill Koch’s postulates for C. luteo-olivacea on Q. robur seedlings and quantify inhibition of Cadophora by a natural isolate of Trichoderma atroviride. Contact-zone proteomics at 4 and 8 days post-contact reveals a staged, interface-localized antagonist
ic program dominated by fungal cell-wall targeting hydrolases (including chitinases and β-1,3-glucanases), secreted proteases, oxidoreductases, transporters and small secreted proteins, alongside factors consistent with adhesion and self-protection. Together, these complementary assays provide the first experimental evidence that C. luteo-olivacea is pathogenic on Q. robur and delineate mechanistic signatures of T. atroviride mycoparasitism at the interaction front. This framework links nursery-relevant pathogen screening to mechanistic readouts and informs evaluation of biocontrol potential in a foundation forest species.
HTML_URL: https://proteomecentral.proteomexchange.org/dataset/PXD073144
XML_URL: https://proteomecentral.proteomexchange.org/dataset/PXD073144&outputMode=XML
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