New ProteomeXchange dataset PXD079283

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Dear ProteomeXchange subscriber, a new ProteomeXchange dataset is being announced. To see more information, click here:

https://proteomecentral.proteomexchange.org/dataset/PXD079283

Summary of dataset

Status: new
Identifier: PXD079283
HostingRepository: MassIVE
Species: Phaeodactylum tricornutum
Title: Proteome response of the diatom Phaeodactylum tricornutum to short term shifts in nitrogen source and availability
Submitter: Matthew Monroe
LabHead: Andrew E. Allen
Description: The photosynthetic model marine diatom Phaeodactylum tricornutum was cultured with varying nitrogen sources and availability to elicit shifts in the proteome. Cells were grown to mid-exponential phase on ammonium (880 uM), collected by centrifugation and resuspended in N-free media for 2 hrs, and spiked with nitrate (150 uM) for 90 min. Cells were again collected by centrifugation, washed in N-free media, and resuspended in experimental treatments with no nitrogen (N-) and a nitrogen source (300 uM) provided as ammonium, nitrite, or nitrate. Each experimental treatment was sampled after 15 min, 45 min, and 18 hr. Data was searched with MS-GF+ using PNNL's DMS processing pipeline.

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