New ProteomeXchange dataset PXD076035

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Dear ProteomeXchange subscriber, a new ProteomeXchange dataset is being announced. To see more information, click here:

https://proteomecentral.proteomexchange.org/dataset/PXD076035

Summary of dataset

Status: new
Identifier: PXD076035
HostingRepository: PRIDE
Species: Histoplasma ohiense
Title: Determination of the peroxisome proteome of pathogenic stage Histoplasma capsulatum via Proximity Labeling
Submitter: Peter Brechting
LabHead: Chad Alan Rappleye
Description: During infection, Histoplasma capsulatum exists primarily as an intracellular pathogen of phagocytes where it overcomes host defense mechanisms and replicates in the phagosome. This intracellular proliferation of Histoplasma yeasts requires the function of the fungal peroxisome for multiple, incompletely defined roles, with early pathogenesis functions dependent on the proper trafficking of peroxisome matrix proteins carrying a type 1 peroxisome-targeting signal (PTS1). To better understand the potential roles of peroxisomes during Histoplasma infection, we identified the constituents of the peroxisome proteome of pathogenic phase Histoplasma yeasts through proximity labeling using a peroxisome-targeted TurboID biotin ligase. Comparative proteomics using peroxisome- or cytosol-localized TurboID identified 90 high confidence peroxisome proteins. To validate the peroxisome proteome, a subset of nine putative peroxisome proteins were localized to the peroxisome using fluo
rescent protein fusions. To empirically define which matrix proteins of the peroxisome proteome depend on the PTS1 or PTS2 import pathways, peroxisome proteomes were also defined for Histoplasma yeasts lacking the Pex5 or Pex7 cytosolic PTS1 or PTS2 receptors. This assigned over half of the proteins to either the PTS1- or PTS2-dependent peroxisome import pathways and refined the Histoplasma PTS1 tripeptide consensus sequence. The peroxisomal proteome of Histoplasma yeast during macrophage infection was ultimately determined to provide relevance to Histoplasma’s primary pathogenic niche. The identities of the proteins comprising the peroxisome proteome include several enzymes for canonical peroxisome-localized biochemical pathways (e.g., fatty acid utilization and siderophore biosynthesis) but also represent several potential novel functions for the organelle. The defined peroxisome proteome of pathogenic-phase Histoplasma yeasts, particularly the set of proteins imported via th
e PTS1 import pathway, thus establishes the important foundation for understanding how peroxisomes promote intracellular pathogenesis.

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