New ProteomeXchange dataset PXD055843
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ProteomeCentral Agent
May 13, 2026, 8:27:19 PM (8 hours ago) May 13
to ProteomeXchange
Dear ProteomeXchange subscriber, a new ProteomeXchange dataset is being announced. To see more information, click here:
https://proteomecentral.proteomexchange.org/dataset/PXD055843
Summary of dataset
Status: new
Identifier: PXD055843
HostingRepository: PRIDE
Species: Homo sapiens
Title: USP24 is an ISG15 cross-reactive deubiquitinase that mediates IFN-I production by de-ISGylating the RNA helicase MOV10
Submitter: Adan Pinto-Fernandez
LabHead: Adan Pinto-Fernandez
Description: The interferon Stimulated Gene-15 (ISG15) is a ubiquitin-like modifier induced by type I Interferon (IFN-I) and plays a crucial role in the innate immune response against viral infections. ISG15 is conjugated to target proteins by an enzymatic cascade through a process called ISGylation. While USP18 is a well-defined deISGylase counteracting ISG15 conjugation, ISG15 cross-reactive deubiquitylating enzymes (DUBs) have also been reported. Our study reports USP24 as a novel ISG15 cross-reactive DUB identified through activity-based protein profiling (ABPP). We demonstrated that recombinant USP24 processed pro-ISG15 and ISG15-linked synthetic substrates in vitro. Moreover, the depletion of USP24 significantly increased the accumulation of ISG15 conjugates upon IFN-β stimulation. An extensive proteomic analysis of the USP24-dependent ISGylome, integrating total proteome, GG-peptidome, and ISG15 interactome data, identified MOV10 as a specific target of USP24 for deISGylation
. Further validation in cells revealed that ISGylated MOV10 enhances IFN-β production, whereas USP24 deISGylates MOV10 to negatively regulate the innate immune response. This study showcase USP24's novel roles in modulating ISGylation, IFN-I production, and innate immune responses with therapeutic implications in infectious diseases, cancer, autoimmunity, and neuroinflammation.
HTML_URL: https://proteomecentral.proteomexchange.org/dataset/PXD055843
XML_URL: https://proteomecentral.proteomexchange.org/dataset/PXD055843&outputMode=XML
https://proteomecentral.proteomexchange.org/dataset/PXD055843
Summary of dataset
Status: new
Identifier: PXD055843
HostingRepository: PRIDE
Species: Homo sapiens
Title: USP24 is an ISG15 cross-reactive deubiquitinase that mediates IFN-I production by de-ISGylating the RNA helicase MOV10
Submitter: Adan Pinto-Fernandez
LabHead: Adan Pinto-Fernandez
Description: The interferon Stimulated Gene-15 (ISG15) is a ubiquitin-like modifier induced by type I Interferon (IFN-I) and plays a crucial role in the innate immune response against viral infections. ISG15 is conjugated to target proteins by an enzymatic cascade through a process called ISGylation. While USP18 is a well-defined deISGylase counteracting ISG15 conjugation, ISG15 cross-reactive deubiquitylating enzymes (DUBs) have also been reported. Our study reports USP24 as a novel ISG15 cross-reactive DUB identified through activity-based protein profiling (ABPP). We demonstrated that recombinant USP24 processed pro-ISG15 and ISG15-linked synthetic substrates in vitro. Moreover, the depletion of USP24 significantly increased the accumulation of ISG15 conjugates upon IFN-β stimulation. An extensive proteomic analysis of the USP24-dependent ISGylome, integrating total proteome, GG-peptidome, and ISG15 interactome data, identified MOV10 as a specific target of USP24 for deISGylation
. Further validation in cells revealed that ISGylated MOV10 enhances IFN-β production, whereas USP24 deISGylates MOV10 to negatively regulate the innate immune response. This study showcase USP24's novel roles in modulating ISGylation, IFN-I production, and innate immune responses with therapeutic implications in infectious diseases, cancer, autoimmunity, and neuroinflammation.
HTML_URL: https://proteomecentral.proteomexchange.org/dataset/PXD055843
XML_URL: https://proteomecentral.proteomexchange.org/dataset/PXD055843&outputMode=XML
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