New ProteomeXchange dataset PXD025869

[ProteomeCentral Agent]

Dear ProteomeXchange subscriber, a new ProteomeXchange dataset is being announced. To see more information, click here:

http://proteomecentral.proteomexchange.org/dataset/PXD025869

Summary of dataset

Status: new
Identifier: PXD025869
HostingRepository: PRIDE
Species: Simulium damnosum s.l. Me1
Title: Characterization of Simulium damnosum salivary antigens
Submitter: Laura Willen
LabHead: Petr Volf
Description: Simulium damnosum sensu lato (s.l.) blackflies transmit Onchocerca volvulus, a filarial nematode that causes human onchocerciasis. Human landing catches (HLCs) is currently the sole method used to estimate blackfly biting rates but is labour-intensive and questionable on ethical grounds. A potential alternative is to measure host antibodies to vector saliva deposited during bloodfeeding. In this study, the salivary proteome of S. damnosum s.l. was investigated. Blackflies were collected in Ghana by HLCs and dissected to extract their salivary glands. Salivary proteins were separated by gel-electrophoresis, and antigenic proteins visualized by immunoblot. Liquid chromatography mass spectrometry (LC–MS/MS) was performed to characterize the proteome of S. damnosum s.l. salivary glands. Several antigenic proteins were recognized, with the major ones located around 15 and 40 kDa. LC–MS/MS identified the presence of antigen 5-related protein, apyrase/nucleotidase, and hyal
uronidase.

HTML_URL: http://proteomecentral.proteomexchange.org/dataset/PXD025869
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