New ProteomeXchange dataset PXD073890

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Dear ProteomeXchange subscriber, a new ProteomeXchange dataset is being announced. To see more information, click here:

https://proteomecentral.proteomexchange.org/dataset/PXD073890

Summary of dataset

Status: new
Identifier: PXD073890
HostingRepository: PRIDE
Species: Homo sapiens , Plasmodium falciparum (isolate 3D7)
Title: TurboID Proximity Profiling of FIKK10.2 (PF3D7_1039000) in Plasmodium falciparum–Infected Red Blood Cells During Febrile Heat Stress
Submitter: David Jones
LabHead: Moritz Treeck
Description: This project investigates how febrile heat stress alters the local protein environment of the Maurer’s cleft resident kinase FIKK10.2 in the malaria parasite Plasmodium falciparum. During human infection, parasites are routinely exposed to elevated temperatures associated with fever, yet how this physiological stress affects the organisation and trafficking of exported proteins within infected red blood cells remains poorly understood. To address this, we used proximity-dependent biotinylation (TurboID) to map changes in the protein neighbourhood of FIKK10.2 under normal and heat stress conditions. Transgenic parasites were generated expressing TurboID fused to the C-terminus of endogenous FIKK10.2. Highly synchronous (two hours window) asexual blood-stage parasites were exposed to febrile heat stress (39 °C) and compared with parasites maintained at standard culture temperature (37 °C). Biotin labelling was applied either continuously throughout the asexual life cyc
le or as a defined pulse between 16–24 hours post-invasion, with or without heat stress, resulting in three experimental conditions. Infected red blood cells were harvested at 40 hours post-invasion following Percoll density enrichment and lysed in 8 M urea. Proteins were digested to peptides, and biotinylated peptides were selectively enriched using anti-biotin antibodies. Enriched peptides were eluted under acidic conditions and analysed by liquid chromatography–tandem mass spectrometry. The resulting dataset provides a high-resolution view of the FIKK10.2 proximal proteome and, by extension, the Maurer’s cleft protein environment. These data reveal how febrile heat stress influences protein trafficking and local protein composition in P. falciparum, offering insights into temperature-dependent regulation of host cell remodelling during malaria infection.

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