New ProteomeXchange dataset PXD059719

[ProteomeCentral Agent]

Dear ProteomeXchange subscriber, a new ProteomeXchange dataset is being announced. To see more information, click here:

https://proteomecentral.proteomexchange.org/dataset/PXD059719

Summary of dataset

Status: new
Identifier: PXD059719
HostingRepository: PRIDE
Species: Hordeum vulgare , Triticum aestivum
Title: Identification of celiac-disease active peptides in gluten-free beers by nanoLC-MS/MS
Submitter: Eleonora Tissen
LabHead: Katharina Scherf
Description: Barley beers may be rendered gluten-free by specialized brewing processes. One controversial issue is whether these beers contain residual gluten peptides carrying at least one celiac disease (CeD)-active epitope, because routine methods to assess whether the beer is gluten-free like competitive enzyme-linked immunosorbent assays (ELISA) may fail to detect potentially harmful peptides. We used untargeted nano-liquid chromatography-tandem mass spectrometry (nanoLC MS/MS) to identify CeD-active peptides in 21 gluten-free barley beers and four regular or carbohydrate-reducedbeers. While the G12 ELISA confirmed gluten levels below 20 mg/kg in all gluten-free beers, the R5 ELISA detected gluten levels in four beers exceeding this threshold, highlighting method discrepancies. nanoLC-MS/MS identified 44 CeD active peptides in seven gluten-free and four non-gluten-free beers, of which 17 CeD-active peptides had no ELISA epitope and therefore escape detection. Accurate quantitati
on of thesepeptides combined with in vivo toxicity assessment will be needed to provide guidance on clinical relevance.

HTML_URL: https://proteomecentral.proteomexchange.org/dataset/PXD059719
XML_URL: https://proteomecentral.proteomexchange.org/dataset/PXD059719&outputMode=XML