New ProteomeXchange dataset PXD049426

[ProteomeCentral Agent]

Dear ProteomeXchange subscriber, a new ProteomeXchange dataset is being announced. To see more information, click here:

https://proteomecentral.proteomexchange.org/dataset/PXD049426

Summary of dataset

Status: new
Identifier: PXD049426
HostingRepository: PRIDE
Species: Mus musculus
Title: mini-Complexome Profiling, an FDR-controlled workflow for global targeted Detection of protein complexes (Heart Data)
Submitter: Christof Lenz
LabHead: Christof Lenz
Description: Co-fractionation mass spectrometry experiments (CF-MS) using native-like protein separations such as Blue Native electrophoresis (BNE) or size exclusion chromatography (SEC) enable a global characterization of protein networks, and a better understanding of cellular functions. They do, however, pose significant requirements on sample amounts, on wet lab and on instrument time, which frequently renders statistically useful replication or comparative experiments between multiple biological states non-feasible. Here we present a fast workflow called mini-Complexome Profiling (mCP) for global targeted detection of annotated protein-protein complexes. It comprises mild extraction of complexes, fractionation by BNE and analysis by data independent acquisition mass spectrometry (DIA-MS). Of note, BNE fractionation into 35 fractions is achieved using commercial mini-gels, with minimal requirements on sample amounts. Complexes are detected using a novel, bespoke R package with a
controlled false discovery rate (FDR) approach. The tool is available to the community on a Github repository (https://github.com/hugoagno3/mCP ).

HTML_URL: https://proteomecentral.proteomexchange.org/dataset/PXD049426
XML_URL: https://proteomecentral.proteomexchange.org/dataset/PXD049426&outputMode=XML