New ProteomeXchange dataset PXD078458
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ProteomeCentral Agent
Jun 4, 2026, 10:06:07 PM (2 days ago) Jun 4
to ProteomeXchange
Dear ProteomeXchange subscriber, a new ProteomeXchange dataset is being announced. To see more information, click here:
https://proteomecentral.proteomexchange.org/dataset/PXD078458
Summary of dataset
Status: new
Identifier: PXD078458
HostingRepository: iProX
Species: Homo sapiens
Title: Site-specific and Multiplexed Profiling of Bile Acid-Protein Interactions
Submitter: Yicheng Liu
LabHead: Chu Wang
Description: Bile acids (BAs) are a family of metabolites with an identical steroid core and versatile functional groups, which play central roles in digestion, metabolism and signaling. While endogenous receptors for selected BAs have been identified, a global BA-interactome map with site-specific resolution and ranked binding preferences is still lacking. Here, we report a high-throughput chemoproteomic platform named “SPIDER” that integrates ligand-directed release (LDR) chemistry with quantitative proteomics for site-specific and multiplexed profiling of BAs’ interactome. We applied SPIDER to quantify 770 interactions between six common BAs with 493 proteins in proteomes with both binding pockets mapped and binding preferences ranked. We discovered the microbiota-originated lithocholic acid (LCA) as an endogenous inhibitor of hydroxymethylglutaryl-CoA synthase 1 (HMGCS1), a key enzyme in the mevalonate pathway, and developed a potent covalent inhibitor for the enzyme based
on an LCA-mimicking natural product to effectively lower nascent cholesterol biosynthesis. We further extended SPIDER to multiplexed interactome profiling of 19 FDA-approved drugs and established a comprehensive map of protein-drug interactions to guide drug screening and repurposing. These data highlight SPIDER as an enabling tool to streamline site-specific and comparative interactome profiling with diverse bioactive ligands in complex biological systems.
HTML_URL: https://proteomecentral.proteomexchange.org/dataset/PXD078458
XML_URL: https://proteomecentral.proteomexchange.org/dataset/PXD078458&outputMode=XML
https://proteomecentral.proteomexchange.org/dataset/PXD078458
Summary of dataset
Status: new
Identifier: PXD078458
HostingRepository: iProX
Species: Homo sapiens
Title: Site-specific and Multiplexed Profiling of Bile Acid-Protein Interactions
Submitter: Yicheng Liu
LabHead: Chu Wang
Description: Bile acids (BAs) are a family of metabolites with an identical steroid core and versatile functional groups, which play central roles in digestion, metabolism and signaling. While endogenous receptors for selected BAs have been identified, a global BA-interactome map with site-specific resolution and ranked binding preferences is still lacking. Here, we report a high-throughput chemoproteomic platform named “SPIDER” that integrates ligand-directed release (LDR) chemistry with quantitative proteomics for site-specific and multiplexed profiling of BAs’ interactome. We applied SPIDER to quantify 770 interactions between six common BAs with 493 proteins in proteomes with both binding pockets mapped and binding preferences ranked. We discovered the microbiota-originated lithocholic acid (LCA) as an endogenous inhibitor of hydroxymethylglutaryl-CoA synthase 1 (HMGCS1), a key enzyme in the mevalonate pathway, and developed a potent covalent inhibitor for the enzyme based
on an LCA-mimicking natural product to effectively lower nascent cholesterol biosynthesis. We further extended SPIDER to multiplexed interactome profiling of 19 FDA-approved drugs and established a comprehensive map of protein-drug interactions to guide drug screening and repurposing. These data highlight SPIDER as an enabling tool to streamline site-specific and comparative interactome profiling with diverse bioactive ligands in complex biological systems.
HTML_URL: https://proteomecentral.proteomexchange.org/dataset/PXD078458
XML_URL: https://proteomecentral.proteomexchange.org/dataset/PXD078458&outputMode=XML
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