Status: new
Identifier: PXD068054
HostingRepository: MassIVE
Species: Recombinant Protein
Title: Analyzes of PEGylation of a Heterogeneous Therapeutic Protein by Direct Mass Technology and Data Dependent Acquisition
Submitter: Zoltan Szabo
LabHead: Zoltan Szabo
Description: Intact mass of a heterogeneous therapeutic protein was determined by Direct Mass Technology (Orbitrap based charge detection mass spectrometry), proving mono PEGylation of the biologic. Furthermore, using multiple enzyme combinations, major PEGylation sites of the protein were mapped by finetuning instrument parameters and chromatographic conditions.