New ProteomeXchange dataset PXD066046

[ProteomeCentral Agent]

Dear ProteomeXchange subscriber, a new ProteomeXchange dataset is being announced. To see more information, click here:

https://proteomecentral.proteomexchange.org/dataset/PXD066046

Summary of dataset

Status: new
Identifier: PXD066046
HostingRepository: PRIDE
Species: Bacillus subtilis
Title: Bacteria Sense the Antibiotic Rifampicin Through a Widespread Dual-Promoter Based Alarm System
Submitter: Alena Krenkova
LabHead: Libor Krásnı
Description: Most antibiotics are natural compounds or their derivatives, and bacteria have evolved defensive mechanisms to resist them. Many of these mechanisms are still poorly understood or uknown. This study reveals that in Bacillus subtilis the transcription factor HelD increases resistance to rifampicin by protecting its target, RNA polymerase (RNAP). This protection is mediated by the N-terminal domain of HelD that penetrates into RNAP to the close vicinity of the rifampicin binding pocket. Importantly, the bacterium detects low rifampicin levels using a unique regulatory system involving two convergent promoters: inhibition of one activates the other that drives helD expression. A similar two-promoter setup also controls the pps gene, which encodes a rifampicin-modifying enzyme. These findings define a widespread bacterial response system sensitive to rifampicin as this dual-promoter architecture is conserved across many bacterial species and found upstream of genes potential
ly involved in rifampicin resistance, such as those for hydrolases, transporters, and transferases.

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