New ProteomeXchange dataset PXD045648

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https://proteomecentral.proteomexchange.org/dataset/PXD045648

Summary of dataset

Status: new
Identifier: PXD045648
HostingRepository: PRIDE
Species: Homo sapiens , Neisseria meningitidis serogroup C (strain 8013)
Title: Plasma membrane deformation by one-dimensional wetting triggers the formation of a branched F-actin network in endothelial cells
Submitter: Magalie Duchateau
LabHead: Guilllaume Dumenil
Description: Membrane deformation by one-dimensional (1D) wetting, a physical process driven by the interaction of the plasma membrane with adhesive nanofibers, triggers the formation of filopodium-like plasma membrane protrusions. In these protrusions, F-actin reorganization occurs after membrane deformation. Here, we interrogate the molecular mechanisms underlying F-actin reorganization in such protrusions formed by human primary epithelial and endothelial cells. While generally assumed that filopodium-like protrusions encompass F-actin bundles, as we observed in epithelial cells, cryo-electron tomography unexpectedly revealed a branched F-actin network in 1D wetting protrusions formed by endothelial cells. Combining high resolution photonic microscopy approaches with genetic and drug perturbations, we show that Arp2/3 activity is required for the proper reorganization of F-actin in 1D wetting endothelial protrusions, a process regulated through the local activation of Cdc42 and N-
WASP. Our data suggest that F-actin reorganization in response to membrane deformation by 1D wetting is differentially regulated in epithelial versus endothelial cells, possibly impacting a wide range of physiological processes, such as cell migration, adhesion, and microenvironment probing.

HTML_URL: https://proteomecentral.proteomexchange.org/dataset/PXD045648
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