Re: Mygo Pro Real Time Pcr
Agathe Thies
The MyGo Pro ESR real-time PCR instrument provides unmatched performance in a convenient format. Novel Full Spectrum Optics deliver 120 optical channels of fluorescence data from every tube in parallel, with no moving parts, for reliable multiplex PCR providing robust 5 colour (FAM HEX ROX CY5 and CY5.5) detection.
High performance Peltier elements, and solid silver blocks, provide both speed and world leading thermal uniformity. The result is rapid, precise, quantitative PCR and melting point analysis. Advanced algorithms combined with an intuitive user interface support a broad range of applications, operating systems and connection options. Performance made easy...
The MyGo Pro real-time PCR instrument providesunmatched performance in a convenient format.Novel Full Spectrum Optics deliver 120 opticalchannels of fluorescence data from every tube inparallel, with no moving parts, for reliable multiplexPCR.
For users performing HRM based analysis of geneticvariants, the combination of thermal control, opticaldata quality and HRM data analysis of the MyGo Prosystem provide compelling functionality. The systemcan discriminate all classes of SNP, including Class 4SNPs via HRM.
Full Spectrum Optics, combined with advanced matrixdeconvolution technology, enable the simultaneousanalysis of at least 7 different targets in a singlereaction, each monitored with a different colour offluorescent label.
The MyGo Pro comes pre-calibrated for 20 differentfluorescent labels, and the software enables usersto use any dye with an emission maximum between510nm and 750nm. No other qPCR system providesthe user with this level of flexibility.
The MyGo Pro has been designed to last, with very few moving parts, made and tested to exacting standards. The result is a highly reliable instrument, which comes with the option of a 5 year extended warranty.
With heating rates of 5°C per second and cooling rates of 4°C per second the MyGo Pro is one of the fastest systems available. No other system matches this speed with convenient-to-use disposables. The system demonstrates excellent quantitative precision and speed, with 45 cycles of PCR in 33 minutes. Typical qPCR systems take much longer than this to deliver results.
The MyGo Pro software does not require a dedicated computer, so users do not need to find the space, or money, for an additional computer to run the system. In addition, the software enables the user to control multiple instruments, and simultaneously analyse multiple runs, with one computer.
The MyGo Mini S is the world's most compact real-time PCR instrument, with no moving parts, enabling silent operation and maximum reliability. It provides users with great results over a broad range of applications.
For users performing HRM-based. analysis of geneticvariants, the combination of thermal control, opticaldata quality and HRM data analysis of the MyGo Minisystem provide compelling functionality. The systemcan discriminate all classes of SNP, including Class4 SNPs via HRM.
The MyGo Mini system comes with easy-to-use software, and robust, high performance algorithms which provide accurate results from complex data. Automated analysis of complex HRM data makes this powerful technique more accessible to non-experts, and minimizes operator-dependent variability in data analysis.
The MyGo Mini is the world's first completely solid-state real-time PCR machine, with no moving parts. The MyGo Mini has been designed to last, then made and tested to exacting standards. The result is a silent, highly reliable instrument, with the option of a 5 year extended warranty.
The MyGo Mini software can be installed on Mac OS X, Windows, and Linux operating systems. Use your choice of computer and operating system. The MyGo Mini is also compatible with standard 0.1mL low-profile tubes, which are convenient and cost-effective.
The MyGo Mini software does not require a dedicated computer, unlike some competitor instruments. So users do not need to find the space or money for an additional computer to run the system, and they can run the operating system that they are familiar with. In addition the MyGo Mini software enables the user to control hundreds of instruments whilst simultaneously analyzing run data on one computer. No other qPCR system offers these benefits to the user.
MyGo PRO cyclers are multiplex real-time PCR devices that are suitable for a wide range of applications, including life science research, food speciation tests and the determination and quantification of viruses.
Up to 32 samples can be run in 0.1 ml tubes or 8-strip format.
Fast heating and cooling is achieved by utilizing robust Peltier elements, while assay performance is supported further by a heated lid design.
Excitation is provided by high intensity LEDs filtered to provide light at 500 nm that is capable of exciting all fluorophores commonly used in qPCR.
A prism is used to generate spectra from fluorescent emissions. These spectra are imaged using a CMOS camera.
MyGo PRO Software is delivered and easily installed from a USB flash drive. The software has no license restrictions.
When installed in a network, the software can control several MyGo Pro Instruments connected via LAN.
What is the minimum hold time for thermal cycling?
For holds during thermal cycling with no optical acquisitions, the minimum hold time is 3s. For holds during thermal cycling with optical acquisitions, the minimum hold time is 17s.
Can I use disposables from other vendors?
Yes. However, in order to ensure optimal performance, please ensure that any
3rd party disposables:
1) Are mechanically identical to the standard MyGo disposables, to ensure a
good fit in the mounts and correct operation of the heated lid.
2) Provide vapour tight seals when closed.
3) Are optically transparent.
4) Are biocompatible, and free of DNAse and RNAse contamination.
What quenchers can I use in the instrument?
For best results use non fluorescent quenchers for example BHQ. If you need to use fluorescent quenchers, e.g. TAMRA, make sure to add the fluorescent quencher to the list of targets in the sample setup to enable spectral deconvolution.
Do I need to optically calibrate the instrument before using it?
The MyGo Pro comes pre-calibrated for 22 dyes. If the dyes you want to install are pre-calibrated then you do not have to run a dye calibration.
When should I generate a dye file?
We would recommend generating dye files if you are using a dye that is not present on the list of pre-calibrated dyes. In addition, generating dye files can
help with multiplexing or other challenging applications.
Why do some amplification curves disappear when I change from Full to Background normalisation in Auto Quant?
In Full normalisation mode the scale of low intensity amplifications is boosted.
In Background normalization mode the scale of low intensity amplifications is not boosted.
Why have you not made a 96 well plate MyGo Pro?
We have prioritised data quality over the number of wells. The optical architecture which enables this in the MyGo Pro can not be used for 96 wells, because it involves fibre optics interfaced to the sides of all wells. In a 96 well block we cannot physically access the sides of all wells with fibre optics .
Will my instrument run with empty tubes?
Yes. The MyGo Pro does not need PCR reagents to be present before performing a run. However, the MyGo Pro will not run if disposables are not loaded.
Can I change how my samples and targets are set up while my experiment is running?
Yes, the MyGo Pro will acquire data from all 120 optical channels in every well at every acquisition. This means the user can view raw data from all channels in all wells. You are not restricted to data from a channel chosen at the start of a run.
Do the instruments have built in memory?
Yes. This memory provides for a data buffer during runs to reduce the risk that data is lost due to a poor connection between your PC and the instrument.
Please ensure that you save your data on your PC once your run has finished.
Do I need to use ROX as a passive reference in my MyGo instrument?
Some real-time PCR instruments require the use of ROX as a fluorescent passive reference to correct for optical artefacts. Generally, modern instruments do not require the use of passive fluorescent references. The MyGo instruments do not require the use of ROX as a passive reference.
Where is the threshold for determining Cq values in Auto Quant?
Modern methods of determining Cq values are not based on simple thresholds. Modern methods of Cq determination are model based. Auto Quant fits a model of a PCR amplification to the fluorescence data observed. This model fit then enables the estimation of a number of important parameters including Cq values.
Why do some of my amplification curves drop down before rising in Auto Quant?
Correct background removal in Auto Quant relies on correct qualitative calling. Please check that appropriate thresholds are set so that positive amplifications are called positive.
The MyGo Pro ESR real-time PCR instrument provides unmatched performance in a convenient format. Novel Full Spectrum Optics deliver 120 optical channels of fluorescence data from every tube in parallel, with no moving parts, for reliable multiplex PCR providing robust 5 colour (FAM HEX ROX CY5 and CY5.5) detection. High performance Peltier elements, and solid silver blocks, provide both speed and world leading thermal uniformity. The result is rapid, precise, quantitative PCR and melting point analysis. Advanced algorithms combined with an intuitive user interface support a broad range of applications, operating systems and connection options. Performance made easy.
MyGo Pro and MyGo Pro ESR qPCR instruments are mulitplex real-time PCR instruments ideal for a wide variety of applications including life-science research, food speciation testing and viral determination and quantification.
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