[Jyoti Batra]

I have purified a protein from inclusion bodies under denaturing conditions (using 6M GuHCl). Can anybody tell me hw i can renature the protein as i want to use it for antibody production in mice. Is there any method other than dialysis??

[Alina Falero]

It is better renaturing during purification, see the paper:

J

OURNAL OF BACTERIOLOGY, Sept. 2009, p. 5873–5876 Vol. 191, No. 18

doi:10.1128/JB.01206-08

Copyright © 2009, American Society for Microbiology. All Rights Reserved.

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[Paola Mendoza Espinosa]

Hi Jyoti!!

You can see this paper: Insight into "insoluble proteins" with pure water. J. Song/FEBS Letters 583 (2009) 953-959.

I hope can help you

Paola

2011/10/3 Jyoti Batra <jyoti_ba...@yahoo.co.in>

I have purified a protein from inclusion bodies under denaturing conditions (using 6M GuHCl). Can anybody tell me hw i can renature the protein as i want to use it for antibody production in mice. Is there any method other than dialysis??

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