error: ...doesnt overlap any reference sequences

[Courtney Smith]

Greetings,

I aligned these sequences in mothur successfully to the reference fasta I am using within pplacer. Any idea as to why it would align in mothur but then in pplacer the alignment isnt overlapping any of the reference sequences?

Thanks in advance!

Courtney 

[Erick Matsen]

Nope! Have you looked at the sequence alignment?

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Frederick "Erick" Matsen, Associate Member
Fred Hutchinson Cancer Research Center
http://matsen.fredhutch.org/