no query sequences on ref tree after pplacer step

[Huda Ghori]

Hi 

I have succesfully made a tree using pplacer however when I looked at the tree I cant find any of my query sequences. I am attaching the csv file it generated. Please help me. What went wrong and how can I pace my sequences on the reference tree. 

Best, 

[Erick Matsen]

Please read the manual in greater detail.

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Frederick "Erick" Matsen (he/him)
Professor, Fred Hutchinson Cancer Research Center
http://matsen.fredhutch.org/