(A) Stereo diagram of Fe(II)/α-ketoglutarate binding site. The Fe(II) ion is shown as a green sphere, α-ketoglutarate is shown as sticks with carbon atoms in gray and the iron-coordinated solvent water molecule is shown as a small red sphere. (B) Coordination of ent-1PL in the PtlH active site. ent-1PL is shown in sticks with blue carbon atoms and solvent water molecules are shown as small salmon-colored spheres. (C, D) Surface illustration of PtlH colored by electrostatic potential in the absence of helix α10 (c, crystal form II) and with helix α10 shown as cartoon representation to indicate the closure of the active site in the presence of this helix. The bound ent-1PL molecule is shown in blue sticks; bound α-ketoglutarate is shown in gray sticks.
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