annotation on MS/MS spectrum - y0+[s]?

[Rong-Fang Gu]

Hi All,

 

I have got high quality x-linked MS/MS spectrum from pLink search results, but the peaks of MS/MS spectrum were labeled as y0+[s], y1+[s], y2+[s] etc, why it starts with y0 not y1?

 

Thanks,

Rong-Fang Gu 

 

 

[Shengbo Fan]

Hi,

That's just an in-lab habit, which may not that formal. :)

As a supplement, we developed a more powerful tool for the annotation, pLabel, and it is included in the pLink.

The usage is Open pLabel.exe, choose File - Load pLabel file, and choose the *.plabel file generated under the search results.

Hope this will help.

[Matthew Makowski]

Hello,

I'm having a problem using the included pLabel (within the pLink download) to save annotated spectra in batch format ("Save all spectra as"). I can direct pLabel to an output folder, and it iterates over all the pictures and "Saves" them. However, there is nothing in the output folder. Hopefully I am not doing something stupid.

Here is the pLabel file in the pLink_release folder:

[File]

DtaFile=E:\pLink_data\MCM6_Asynch_1\20150130_Q1_SA_PaJa_MM_MCM6gfp_wt_Hela_WCL_xlink_2chargeexcluded.mgf

pLabelFile=E:\pLink_data\MCM6_Asynch_2\2.report\sample1\MCM61_inter.plabel

Sequence=KTDAK-GKFR

PicFile=E:\pLink_data\Pictures\MCM6_Asynch_rep1

DataFile=C:\pLink\pLink_release\DataTemp

[MainInfo]

DissociationMode=0

CrsLink=1

CrsLinkType=3

ReagentSel=0

CrsLinkListSel=1

LineWidth=0

Decimal=1

MatchPeakType=0

MonoAver=1

ShowInfoStatus=1

ShowAASeqStatus=1

ShowMassStatus=1

ShowNLStatus=1

Threshold=1.0

Tolerance=0.500000

ToleranceType=0

PrecursorDevType=0

[Color]

a=64,0,255;0,0,255

b=0,128,0;0,128,0

c=0,128,255;0,128,255

x=255,0,128;255,0,128

y=255,128,64;255,128,64

z=128,0,0;128,0,0

MH=128,128,128

SM=0,255,0

Thanks for any and all help!

Best,

Matthew

[Matthew Makowski]

I should note; I can save spectra individually by this method, but the batch "Save all spectra as" does not actually save any spectra.