Hi, what do you mean by 1/25 ? You should aim for a displacement around 2 to 8 pixels or so. Large interrogation areas are good and will only limit your resolution.
Multiple passes are always better and have less noise and bias. What do you mean by multiple channels?
Thus is my latest paper on PIVlab:
Thielicke, W., Sonntag, R. (2021) Particle Image Velocimetry for MATLAB: Accuracy and enhanced algorithms in PIVlab. Journal of Open Research Software, 9: 12. DOI:
https://doi.org/10.5334/jors.334