Error in PINE server submission

[Jennifer Zhao]

Dear Woonghee,

I have tried a few times to run a job but I keep getting an error. Understandably my protein is 300 residues but on the HSQC I can really only pick around 170 residues. Is there any way around this issue?   

Here is the email I've received: 

% Error: The quality of data is not acceptable. 
 There might be different reasons for this error however the most common ones are: 
 1- The experiments does not align with each other (unacceptable shift in different experiments).
 2- There are unacceptable number of noise in experiments.
 3- One or more of the uploaded files is different from the experiments supposed to be uploaded in that field.
    e.g.: uploading CBCA(CO)NH experiment in HNCACB field, etc

Thank you very much, 

Jennifer 

[Lee, Woonghee]

Dear Jennifer,

If you have good connectivities for 170 residues, it will work. I suggest you using strip plots to add peaks not seen in HNCACB but seen in different spectra at the same location. If you have large dynamic ranges at the tail, excluding the sequence file is another way. If none of them works, you can use versatile assigner instead. Check out youtube videos for the versatile assigner usage.

Best,

Woonghee

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From: 'Jennifer Zhao' via I-PINE/PINE and ssPINE user group <pinenmr-u...@googlegroups.com>
Sent: Monday, July 19, 2021 11:03:20 PM
To: I-PINE/PINE and ssPINE user group <pinenmr-u...@googlegroups.com>
Subject: Error in PINE server submission

 

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[Jennifer Zhao]

Thank you Woonghee, I'll try that.