Brunello library sequences

[Ruud Wijdeven]

Good morning,

I'm very happily using PinAPL for my screening analyses, using both custom as well as non-custom libraries. However, for the Brunello library I'm wondering whether the order on the addgene page is the order of the sequence numbers, because I sometimes have gRNAs that appear from the screen but don't give a knockout. 

Is is possible to send the gRNA sequences together with the numbers used in the PinAPL pipeline?

Thank you very much!

Ruud Wijdeven

[Anne Maglott]

Hello 

Did you receive the corresponding file between Addgene sequence and BRUGENnumber used in PinAPLPy?

I will be interested of having it as well.

Thank you

Anne

[Ryan Weiss]

Hi all,

Apologies that no one got back to you on this sooner. I had a similar question about differences between BRUGEN IDs and the original sgRNA library file from the Brunello library publication. I have obtained the original sgRNA file used for PinAPL-Py analysis from the developers. This file contains the BRUGEN numbers for sgRNAs for the Brunello library (see attached). You can open the attached file in Excel. 

Hopefully this helps!

-Ryan