Server maintenance? and request for library sgRNA sequences

[Jianfeng Lin]

Good morning,

I am having issue with uploading my fasta.gz files from last afternoon. I realize that it could be the server maintenance or the my internet problem. Thus, i would like to confirmed with you to see if the PinAPL-Py server is undergoing maintenance? 

At the same time, it would be greatly appreciated if you could send me the sgRNA sequences for the Human Brunello whole genome library file. While i have the full list of the sgRNA sequences from Addgene.com, it seems like the sgRNA ID results that i got from this analysis could not be identified in my file. I therefore would like to request for the library file that you have.

Thank you in Advance.

Regards,

Jianfeng

[Ryan Weiss]

Hi Jianfeng,

Apologies that no one got back to you on this sooner. Attached you will find the sgRNA list and IDs used for PinAPL-Py analysis for the Brunello library (see attached). You can open the attached file in Excel. 

Hopefully this helps!

-Ryan