Error: No control treatment defined!

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Devin Dersh

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Apr 28, 2023, 2:21:44 PM4/28/23
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Hello, I'm trying to run an analysis but each time the output log gives:

### ERROR: No control treatment defined! ###

I certainly checked off the control samples. When I look at the Sample Names tab, none of the labels that I typed in during the setup seem to be showing up, as if they are not recording the sample names at all. 

Any thoughts? Thanks so much
Devin


Gaia Lupoli

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May 3, 2023, 7:33:00 AM5/3/23
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Hi Devin,

I actually had exactly the same error message last week and I also had for sure checked off the control samples.
I'm wondering if PinAPL-Py has some sort of bug.
Today I'm again trying with renamed files (the control duplicates didn't have the same name before and this could be an issue).

Did you find a solution to your problem?

Gaia

Devin Dersh

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May 3, 2023, 3:55:21 PM5/3/23
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Hi, I submitted a bug report and also uploaded the demo data files to see if that would work. I suspect there was a server restart or something because after several days of being stuck in queue, the demo data files ran and did NOT display the "no control treatment defined" message. I will run my real samples again now to see. But hoping it was just an issue with the server. Let's see.

Devin Dersh

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May 6, 2023, 10:15:08 AM5/6/23
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I am still trying to troubleshoot this but did you concatenate your fastq files going in? When I stopped doing that, it seems like the runs are succeeding. Like I said, still trying to figure it out, but I don't think it's a general issue with the program...
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