Is there a way to do full length 16s functional analysis with picrust2

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David Liu

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Jul 7, 2025, 11:34:17 AMJul 7

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Hi,

I am trying to do functional analysis with full length 16s sequences, do you have any idea how can I use picrust2 or any other tools?

Thanks!

David

Robyn Wright

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Jul 7, 2025, 1:14:26 PMJul 7

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Hi David,

You will be able to run PICRUSt2 exactly the same way with full-length 16S sequences as with shorter regions of the 16S rRNA gene. You'll need to process your samples to the point that you have a file of representative sequences and a feature table containing their abundances within your samples prior to running PICRUSt2 - we have a QIIME2 pipeline that you can follow here if you like.