Help with picrust2 using Mothur

[Allan Amorim Santos]

Hi everyone, hope you are doing well.

I'm a beginner user and I'm working on Mothur for analysing my v4 sequencing data.

Following my experimental setup, we would like to predict the functions by using picrust2.

Basicaly, I was reading that I need fna file (conventional fasta file) and biom file; however I'm not sure how to proceed with biom file using Mothur.

After generating biom file following the commands, I found some errors in picrust2 since OTU/ASV id is not the same in fasta and biom.

Please, could anyone help me with this?

Thank you so much!!