Hi,
I am looking for a way to get functional analysis with 16s long reads. For the abundance classification, I am using the Kraken2 tool. And then for the biome file, I am using the Kraken biome and generating a biome format table with the first column as taxaID and other columns for sample abundance.
but I am unable to generate an ASV file format table and an ASV_ID associated sequence fasta file which are needed to picruste2 as the input file.
Is there a way I could go ahead with doing functional annotations with current data?
Or is there any other way to generate ASV file from nanopore sequencing data?
Any help is appreciated!
Thanks in advance!