about picrust for metagenome

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Aug 20, 2024, 3:29:46 AM8/20/24

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Hi, can we use Picrust for whole genome metagenome, If then how can we use this on for metagenome

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Aug 23, 2024, 10:03:37 AM8/23/24

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Hello,

PICRUSt is for creating predictions of what would be in the meta genome. If you already have the metagenome sequenced then you can just annotate the metagenome - no predictions needed. You can see the workflow that we use for this in our lab here: https://github.com/LangilleLab/microbiome_helper/wiki/Metagenomics-Standard-Operating-Procedure-v3 Using HUMANN for this is another option.