Hi,
I am performing PICRUSt analysis for a very first time, trying to use q2-picrust2 with my environmental samples (sludge samples).
Here is command I have used:
qiime picrust2 full-pipeline \
--i-table merged-table_EF_RW.qza
--i-seq merged-rep-seqs_EF_RW.qza
--output-dir q2-picrust2/q2-picrust2_output
--p-placement-tool epa-ng
--p-threads 32
--p-hsp-method mp
--p-max-nsti 2
--verbose
In response of the above command I was getting this warning.
Warning - 2 input sequences aligned poorly to reference sequences (--min_align option specified a minimum proportion of 0.8 aligning to reference sequences). These input sequences will not be placed and will be excluded from downstream steps.
This is the set of poorly aligned input sequences to be excluded: 8fad6efc85152fc02447ff4a180b8ad9, 02b86a50afd72eee48f6669b5aa67fa9
What it means????Can I use these results?
**43 of 3158 ASVs were above the max NSTI cut-off of 2.0 and were removed from the downstream analyses.
**43 of 3158 ASVs were above the max NSTI cut-off of 2.0 and were removed from the downstream analyses.
Please suggest whether, these values are of positive interest or not??? If not, then how can I play with parameter **
--p-max-nsti 2
Any suggestion or comment will be appreciated.
Best Regards,
Rashmi