query_align.stockholm empty file

[A Cupples]

The first step (place_seqs.py) only generated an empty file called

query_align.stockholm.

My fasta file is 400MB, is this too big?

Thanks

[Gavin Douglas]

Hi there,

How much memory do you have? The default placemethod method (EPA-NG) needs at least 16 GB of RAM. You could try using the “sepp” placement method, which might work better for your dataset.

Cheers,

Gavin

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