ANCOM analysis issues with picrust2 in Qiime2 plugin

[mashu...@gmail.com]

Hi all,

I have been trying to run ANCOM analysis with picrust2 in Qiime2

First run:

  (qiime2-2019.10) msiddiq7@enggpz1p23:~/Kang_dataset_picrust$ qiime composition add-pseudocount \
>   --i-table ko_metagenome_kangdata.qza\
>   --o-composition-table ko_metagenome_kangdata_added.qza
Saved FeatureTable[Composition] to: ko_metagenome_kangdata_added.qza

Second run:
(qiime2-2019.10) msiddiq7@enggpz1p23:~/Kang_dataset_picrust$ qiime composition ancom \
>   --i-table ko_metagenome_kangdata_added.qza \
>   --p-transform-function log \
>   --m-metadata-file metadata_mclrkd.tsv \
>   --m-metadata-column Group \
>   --o-visualization ANCOM_mclrkd.qzv

Saved Visualization to: ANCOM_mclrkd.qzv  

Problem 1: When I have tried to visualize the qzv file in Qiime2view, it showed an error (Screenshot is attached ANCOM_viz_q2v.jpg).

  Problem 2: 

Following this, I have tried to run ANCOM analysis by selecting other metadata column (classification):

​(qiime2-2019.10) msiddiq7@enggpz1p23:~/Kang_dataset_picrust$ qiime composition ancom \
>   --i-table ko_metagenome_kangdata_added.qza \
>   --p-transform-function log \
>   --m-metadata-file metadata_mclrkd.tsv \
>   --m-metadata-column classification \
>   --o-visualization ANCOM/ANCOM_cls_mclrkd.qzv

This command has been running overnight but showing nothing (showed in the attachment ANCOM_running.jpg).

Any suggestion to resolve the issues will be really appreciated.