Picrust warning output

[Muhammad Fikri Alfiansyah]

Hello, I got this message after running picrust2 full pipeline, I have matched the IDs from the input files and reached 99.9% the only mismatch is the #OTUID part

28 of 31952 ASVs were above the max NSTI cut-off of 2.0 and were removed from the downstream analyses.
Warning: fewer than half of the sequence ids overlap between the input files.

[Robyn Wright]

Hello,

The first line isn't an error message, it is simply PICRUSt2 letting you know that some of your ASVs were not similar to those in the reference database and were removed from predictions. 

The second line is warning you that not all of your sequence IDs overlap between the fasta file and your feature table. All of the ASV names in the OTUID column should match sequences found in the fasta file.

If you have any other issues, please see the FAQ page and submit an issue on Github with all information listed in the bug report template. 

Thanks,

Robyn