ASV cutoff for input files in picrust2

[ROHIT PATEL]

Working with Picrust2, I have ASVs and their abundance converted into percentage values. So when selecting which ASVs should be included in the Picrust2 input biom file, what are the criteria. 

1. Is there any percentage value to determine the cutoff. For instance, organisms with more than or equal to 5% abundance in at least 1 sample need to be used in analysis, whereas organisms with less than 5% abundance across all samples should be discarded. as they could produce faulty results.

[Ricardo Rocha]

Hi Rohit,

If I'm not wrong, for the Picrust the input should be an ASV table without transformation or filtering. 

Cheers

Ricardo

On Wed, Jun 21, 2023 at 2:08 AM ROHIT PATEL <rohitmicr...@gmail.com> wrote:

Working with Picrust2, I have ASVs and their abundance converted into percentage values. So when selecting which ASVs should be included in the Picrust2 input biom file, what are the criteria. 

1. Is there any percentage value to determine the cutoff. For instance, organisms with more than or equal to 5% abundance in at least 1 sample need to be used in analysis, whereas organisms with less than 5% abundance across all samples should be discarded. as they could produce faulty results.

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