I've run PICRUST2 using the MetaCyc reference database on my bacterial 16S sequencing data.
When I looked at the output some pathways were related to methanogenesis (methanogenesis from acetate and METHANOGENESIS-PWY).
However, this pathway is only found in the Archaea Kingdom, right?
So, I'm wondering if anyone has any clues about what might have happened.
Before Picrust2 I removed all the sequences related to Archaea or unknown taxonomy.
Just to let you know, I used DADA2 for the sequence analysis.