we may be on to something

[Jonathan.Lake]

Hey team, Just wanted to share this tid bit with you. The "doubling effect I was referring to last night is called multiphonton microscopy. And I found an article last night that describes using it exactly for our purpose see attached article. Also I have attached a review article from 2012 that says this type of imaging is promising new technology in the area of nerve sparing during prostatectomy. This being used in concert with the "real time" FLIM imgaging we already know about could be the exact technology we are looking for.

Best,

--
Jonathan Lake
Sensors and Technology Lab
Department of Electrical Engineering
University of California, Los Angeles
Tel. (818)-470-1835
Email: jonath...@ucla.edu

[Zach McKinney]

Hey Jon -

Thanks for the great info.  Better excitation wavelength and greater penetration depth would definitely be a game changer. 

As for the laparoscopes, I asked Dutson said that if properly treated, laparoscope seldom (if ever) need to be replaced, but that they're often not so well treated. He forwarded the inquiry to his department's buyer for an estimate of how often they replace the scopes on practice, as well as what they pay for them.  I'll forward you more info as soon as I have it (and an Internet connection).

Until then, 

-Zach-->

<multiphoton microscopy applied to nerve visualization during prostatectomy.pdf>

<new technologies under development.pdf>

[Peter Borgstrom]

Very exciting stuff Jon. We can talk more on Monday, but a few things about the article that stuck out:

1. From my readings, Tewari is one of the biggest names in robotic prostatectomies (his site is where we got a lot of pictures/videos from), so this is a very legit group working on this.

2. Its really helpful to know that they used rats, and claim this to be a "well-recognized model" for this very purpose. If we are going to propose an animal model, rat should probably be it.

3. I really like their idea of taking both low resolution images for "overall architecture information" and high resolution for cellular and local architecture. I'm not sure how this would work in-vivo, but its something to think about. 

4. They actually use 740-780nm lasers that they say excite intrinsic fluorophores in most living cells. So we might want to also try with just the 830 nm light without the doubling effect to see what that gives us, since thats closer to this range.

  

5. Their second harmonic generation signal (which if I understood correctly is the analog of our second image) was collected at 390 +-35 nm, which is consistent with our best contrast coming around 407 nm. 

Great finds on brother and papers Jon!

Peter 

[Thomas Rogerson]

Wow! Just read all these emails - very exciting stuff.

I will have the mice ready for dissections next week. If your brother wants to do the imaging Wednesday, Thursday or Friday we maybe should plan on getting the samples on Wednesday. It turns out my flight leaves pretty early Thursday so that day wont work for me.

Thomas

[Jonathan Lake]

Sounds good for me. Ben will that work for you anytime wed?

Sent from my iPhone

[Thomas Rogerson]

I just checked the availability of the room we do dissections in and we will need to do it in the morning.

I hope that is OK with you Ben and Jon? We could also do the dissection after our class but you would then need somewhere to keep the samples until you want to image them.

Thomas

[Ben Kelley]

Hey guys,

Sorry for the late notice but I won't be able to make tonight's meeting.  I'm free for dissection Wednesday after class if that's still the plan, Thomas and Jonathan.

Best,

Ben

Sent from my iPhone

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[Thomas Rogerson]

That works for me. We will just have to pack then in a lot of ice or find a fridge you guys can keep them in until you image on Thursday. 

See you in class,

Thomas

[Jonathan Lake]

What time was that again? Sounds great!

Sent from my iPhone

[Thomas Rogerson]

Whenever class gets done. I'm guessing 7-8pm Wednesday.

Thomas