FLIM CNSI update

[Jonathan.Lake]

Hey team,

So I wanted to update you all on what I just learned. So switching the excitation wavelength on Asael's system is definitely possible but is kind of a long term project(may not be worth it at this stage). 

    The good news however is that my brother just happens  to work here at UCLA in CNSI on FLIM imaging in the Advanced Light Microscopy and Spectroscopy Lab(believe it or not) and he says we can bring him samples to try out next week. Not only can we try different excitation wavelengths but we will extract full lifetime decay times which should give us the best contrast that the physics will allow (not a real time technique). The icing on the cake here is that he will actually use 830 nm light to excite the fluorophores and use a doubling effect (two photons arriving at the same time) to excite fluorophores at around 415 nm.  The big advantage here is that because we use 830 nm light not  the usual 415 nm light we get a penetration depth on the order of mms NOT just surface imaging.  While there are still plenty of validation and kinks to work out here, there is no fundamental reason we could not use the same tricks on a setup like Asael's to get Quasi real time images using this doubling technique with good penetration.   This is a potential game changer for us so I am very excited that We have the potential to try this out.

  

Just an FYI I will likely be using a couple hundred dollars from our funds for microscope time at CNSI.  Thomas, the plan right now will be to image next week on Wednesday, Thursday, or Friday.  as it gets closer we should find a time to do the dissection(preferably with Ben as well), I believe we will have time to image around 3 samples on my brother's setup (I'm thinking facial nerve network, Prostate, and femoral Nerve at lower bifurcation).

Do not ask me why I did not connect these dots earlier in this process. My brother is a very smart guy, but can be hard to pin down sometimes. lol.

Anyway, I really feel this breathes new life into our efforts. So I hope we all can redouble our efforts and enthusiasm so we can push this thing as far as we can.

Quick recap of tasks

Peter- look into the market analogy to Viagra to make a case for how much men are willing to pay out of pocket for erectile function.

Zach-Find out how much laparascopes cost and how often they need to be replaced.

Ana-set up framework for the Business plan for Monday (side note: I did some quick estimates of how much I thought we could charge for this ability in comparison to the selling price of the system and I would like to discuss some of these basic financials with you at your earliest convenience or next meeting at the latest)

Thomas and Ben- get prepared for next weeks dissection study up on mouse anatomy and surgical techniques to access the prostate cleanly

I look forward to finishing up strong!

Best,

--  
Jonathan Lake
Sensors and Technology Lab
Department of Electrical Engineering
University of California, Los Angeles
Tel. (818)-470-1835
Email: jonath...@ucla.edu

[Ben Kelley]

Awesome news, thanks for the update Jonathan.

Sent from my iPhone

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