Biom file will not load correctly

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pfa...@gmail.com

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Mar 3, 2015, 11:22:04 AM3/3/15
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Hello,

I am having trouble getting my biom file to load correctly. The screen that comes up after I select to load the file is completely blank except for a column of the left that shows my experimental treatments. When I select "proceed to gallery", I can see results on every page  except for attributes. However, some of my files have been named correctly while others are just given numbers. I will attach the biom file I am using so that you can see what is going on for yourself. 

Any help you could provide would be of assistance. 
16s_paired_SDS.biom

Holly Bik

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Mar 4, 2015, 4:36:27 AM3/4/15
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I tested the file and confirmed it wasn't working - not sure why the sample list isn't showing. I've had similar problems before and have been unable to pinpont the problem - I suggest converting your BIOM file back to a classic OTU table, and then re-converting back to BIOM (maybe some field got changed during the initial generation that is interfering with Phinch). Commands to run are as follows:

biom convert -i original_file.biom -o classic_OTUtable.txt -b --header-key=taxonomy

biom convert -i classic_OTUtable.txt -o new_BIOM_file.biom -m mapping_file.txt --table-type="otu table" --process-obs-metadata=taxonomy

biom add-metadata -i new_BIOM_file.biom -o new_BIOM_file_with_metadata.biom -m mapping_file.txt

Then try to load in Phinch again - Let me know if this works!

Haley Pfaffe

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Mar 4, 2015, 11:46:44 AM3/4/15
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Thank you for getting back to me so quickly Holly! Your suggestion worked perfectly!! 

Thanks again,
Haley
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