Plant Tissue Culture Books Pdf

[Elwanda Menhennett]

Asa content and community manager, I leverage my expertise in plant biotechnology, passion for tissue culture, and writing skills to create compelling articles, simplifying intricate scientific concepts, and address your inquiries. As a dedicated science communicator, I strive to spark curiosity and foster a love for science in my audience.

Tissue culture is a technique used to grow plants under in vitro or lab conditions. The technique uses a few tissues or single plant cells to develop them into a whole plant. It utilizes the totipotent property of the plants, where each plant cell has the ability to transform and form any plant cell.

In this article, we will talk about 5 books that provide you with all the basic to advanced information on tissue culture. You can either choose one or read them all to strengthen your tissue culture knowledge.

If you are a beginner in the tissue culture area and want to test some plants in tissue culture. Then, these books can be proved resourceful for you. You can try out the plans and their procedures given in the book to practice tissue culture on your chosen desired plants.

The first edition of the book was published in 1983. But, since then many revisions have taken place to incorporate the discoveries and developed concepts. The book contains the basics of tissue culture, including theory, practicals, and some applied concepts in the area.

If you want to start learning from the zero of tissue culture, then this book is best to introduce you in the best way possible to the tissue culture technique. It is full of illustrations, figures, diagrams, and photos that help you better understand the concepts.

The book contains around nineteen chapters covering topics ranging from the history of tissue culture, media preparation, haploid production, Variant Selection, and protoplast isolation, to germplasm storage.

Plant tissue culture has become an integral part of agriculture biotechnology. And, Introduction to Plant Tissue Culture is a book that talks about the advancements in basic techniques, clonal propagation, and haploid and triploid production. All the concepts are combined with diagrams and illustrations for a better understanding of the concept.

The book is suitable for students and teachers both as it contains experiments and concepts that can be easily executed in labs all year round. The book has step-by-step protocols to tissue culture a range of plants, complemented with many detailed line diagrams.

The book has everything on tissue culture, ranging from protoplast culture, cell suspension cultures, haploid techniques, primary metabolism, phytohormones and growth regulators, and applications of plant tissue culture systems.

It has methods ranging from general methodologies, such as culture induction, growth and viability evaluation, and contamination control, to such highly specialized techniques as chloroplast transformation involving the laborious process of protoplast isolation and culture.

Plant Cell Technology is helping tissue culturists worldwide by providing unique and world-class products and services that smoothen their process. It has MS media, agar, gellan gum, Plant Preservative Mixture (PPM), culture vessels, Biocoupler (TM), and masks in its store to facilitate your processes.

1. History of plant tissue culture2. Setup of a tissue culture laboratory3. Media components and preparation4. Explant preparation5. Contamination6. Callus induction7. Regeneration and morphogenesis8. Woody shrubs and trees9. Haploid plants from another culture10. Embryo rescue11. Meristem culture for virus-free plants12. In Vitro propagation for commercial production of ornamentals13. Protoplast isolation and fusion14. Agrobacterium-mediated transformation of plants14-1. Petunia or tobacco leaf disk14-2. Petunia shoot apex14-3. Tobacco leaf infiltration14-4. Arabidopsis floral dip transformation14-5. Dicot plant-based techniques and experiments14-6. Rice transformation techniques and experiments15. Genome edition and RNAi15-1. Tomato genome edition15.2. Lettuce RNAi

In the first three chapters, the history, terminologies, and applications are given in detail. The fourth chapter is dedicated to the instrumentation of plant tissue culture. The basic techniques used in PTC are described in the sixth chapter. The details of the constituents and types of different nutrient media are discussed in the eighth chapter. In chapter number 9, methods of haploid production have been described. Bioreactors are the instruments that are used for the large-scale production of plantlets and plant products.

Plant micropropagation is an important, and often necessary, technique for cryopreserving vegetatively-propagated plants. Plant tissue culture is a fast and effective way to multiply plant tissues and cells to produce a desired product. Shoot cultures are a vital source of explants for many shoot tip cryopreservation methods and shoot tips are most often pretreated and recovered using an artificial nutrient medium. Plant tissue culture media formulation and preparation are not difficult but does require some planning and special equipment.

When planning to prepare plant tissue culture media, some consideration must be given to purpose of the tissue culture effort, the species of plant being cultured and the intended results. The stages of plant tissue culture are: selection/preparation (stage 0), initiation/establishment (stage 1), multiplication (stage 2), rooting (stage 3), and acclimatization/hardening (stage 4). A formulation for growth medium is selected based on which stage the plant is in or will be entering. Generally it is best to do a literature search, such as from a book or scientific journal articles, to see if there is information available regarding the target plant of interest, suggested media for that plant, and the results obtained.

Some reference books that are relevant to plant tissue culture are: Plant Propagation by Tissue Culture by Edwin F. George, Michael A. Hall, and Geert-Jan De Klerk; Plant Tissue Culture: Techniques and Experiments by Sunghun Park; Plant Tissue Culture: An Introductory Text by Sant Saran Bhojwani and Prem Kumar Dantu; and Plants from Test Tubes: An Introduction to Micropropagation by Lydiane Kyte, John Kleyn, Holly Scoggins, and Mark Bridgen.

There are many components and additives that can be used in plant micropropagation media, but most can be placed into eight categories: water, nutrient salts (micro and macronutrients), vitamins, amino acids, carbohydrates, gelling agents, growth regulators (hormones), and other organic supplements. The following is a summary of each component category:

Antioxidants and antioxidant-like compounds are sometimes added as a supplement to plant tissue culture media to prevent or minimize reactive oxygen species (ROS) that can cause oxidative damage to plant tissues. Ascorbic acid, citric acid, glutathione (reduced form), lipoic acid, glycine betaine, D-tocopherol (vitamin E), salicylic acid, and polyvinylpyrrolidone (PVP) have all been shown to have potential to inhibit ROS formation, but their effects vary widely between different plant species. At the National Laboratory for Genetic Resources Preservation, in Fort Collins, CO, Vitis single-node microcuttings and shoot tips are treated with a mixture of 1 mM ascorbic acid, 1 mM glutathione (reduced form) and 0.1 mM salicylic acid in the pretreatment and preculture media, respectively (Bettoni et al. 2019). This treatment has been shown to significantly improve shoot regrowth after the cryopreservation process, likely due to its antioxidant effects. Similarly, Uchendu, et al. (2010) found that adding either glutathione (reduced), lipoic acid, or glycine betaine to varying steps of the cryopreservation process significantly increased regrowth of cryopreserved shoot tips.

* Media recipes typically include a target pH that will allow the gelling agent to solidify and promote maximal growth for the species in culture. The pH of the medium will likely change (usually a decrease) after autoclaving, especially in the presence of a carbohydrate source such as sucrose. The pH change will vary based on the medium being prepared, different autoclave models, sterilization times, etc., so it is advised to test the pH of the medium both before and after autoclaving to determine how much change has occurred. Next time the medium is prepared, the pH of the medium is then adjusted prior to autoclaving to compensate for the change that was observed previously.

** Commonly used vessels for plant tissue culture include test tubes, Petri dishes, Magenta GA7 vessels, glass jars, and Star*Pac bags. The type of container and volume of medium should reflect the space needed for the plant material to grow before it must be repropagated. Test tubes are especially useful for culture induction since their small volume is suited to segregating explants, thus reducing loss from contamination. Since Petri dishes are shallow, wide, and easy to work with, they are ideal vessels for short-term use of small tissues, such as preconditioning nodes or shoot tips prior to cryopreservation. Both test tubes and Petri dishes come in a variety of sizes; regardless, media should typically occupy less than half the maximum volume of the vessel. Keep in mind that polystyrene Petri dishes are NOT autoclavable and so they must be filled after media sterilization in a laminar flow hood. Magenta vessels and glass jars are ideally suited to multiplying cultures that grow well at higher densities; these are typically filled to less than 15% capacity (for instance, 80 mL medium in a 575 mL Magenta GA7 vessel) to allow ample room for growth. Star*Pac bags may be used to save space under slow growth conditions.

There are many variations of plant tissue culture media based on the desired growth and nutrient requirements of the target plant and how the plant products will be used. There are solid, semi-solid and liquid versions of culture media based on the type of culture and plant species. Many shoot cultures are grown on solidified media to assist in supporting the plant structures as they develop during the growth phase. Other cultures, such as root or suspension, may be cultured in a liquid medium and shaken to provide the proper aeration to the tissue or cells.

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