Thimble Kill 1.0 Zip File Download Free [WORK]
Aline Lanosga
That's why I used read, and then converted that to a string, instead of just picking up the text in the echo area directly. IOW, in that specific case more than just the value is inserted in the echo area - more than what we want copied to the kill-ring.
Exponentially growing cells were irradiated at room temperature with X-rays and C-ion beams. X-irradiation was delivered by a 200-kVp X-ray generator (TITAN-225S, Shimadzu, Kyoto, Japan) with a total filtration of 0.5 mm aluminum plus 0.5-mm copper. The X-ray dose rate was about 1.3 Gy/min which was measured using a thimble ionization chamber (PTW FREIBURG, Freiburg, Germany) at the sample position. C-ion beam irradia- tion (290 MeV/nucleon, 6-cm spread-out Bragg peak (SOBP): 50 keV/μm) [17] was performed in the Gunma University Heavy Ion Medical Center (GHMC, Gunma, Japan) [22] . Each irradiation was performed through a 3.5 mm layer of culture medium and a 1 mm plastic cover in a NuncTM flask or multidish (Thermo Fisher Scien- tific, Waltham, MA).
This study clearly demonstrated that C-ion beams efficiently induced cell killing in X-ray-resistant cells (Figure 1). These findings agree with previous results published by our group [31] -[33] which demonstrated that the RBE of heavy-ion beams in X-ray resistant p53-dysfunctional cells was higher than in X-ray sensitive wild-type p53 cells. Apoptosis was induced more efficiently when cells were exposed to heavy-ion beams than to X-rays, regardless of p53 gene status [31] -[33] . In SAS and SAS-R cells, although the contribution of apopto- sis to cellular radiosensitivity is lower than previously thought [6] , hyperinduced pyknosis was detected and was greater in C-ion irradiated cells than in X-irradiated cells at iso-doses (Figure 2(c)). Pyknosis is the irreversible condensation of chromatin in the nucleus of a cell undergoing apoptosis [11] . On the other hand, polyploid cells were present at lower levels in C-ion irradiated cells than in X-ray irradiated cells at iso-survival doses (Figure 2(d)). Radiation-induced polyploid cells usually suffer cell death from mitotic catastrophe [34] . Mitotic catas- trophe has been described in which mitotic failure is sensed and the cell responds by following a pathway to cell
C-ion beams may simultaneously kill both non-CSCs and CSCs; consequently, the cell population of CSCs was only slightly increased or unchanged (Figure 5). If CSCs were eliminated, a cancer would be unable to grow and unable to spread to other locations in the body. Recently, it was reported that C-ion beams targeted cancer stem-like cells in the colon and pancreas [40] [41] .