Data Matrix in GEO

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Eduardo Gade Gusmão

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Mar 29, 2017, 10:35:45 AM3/29/17
to Perturb-seq
Dear sir/madam,

I wonder whether you applied any of the processing steps discussed on the paper in the UMI expression matrix available in GEO.
To put the question more simply: The GEO UMI matrix contains which pre-processing steps?

Thanks!

Eduardo

Atray Dixit

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Mar 29, 2017, 10:43:01 AM3/29/17
to Perturb-seq
Hi Eduardo,

The GEO UMI matrix contains no additional preprocessing beyond what is intrinsically part of the 10X cellranger pipeline (i.e. UMI collapsing and filtering for the most abundance cell barcodes)

Best,
Atray
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